[Pharmwaste] estrogens light up genetically modified fish

Thu, 10 Mar 2005 12:58:57 -0500

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Technology News -

March 9, 2005

Estrogens light up genetically modified fish

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Masato Kinoshita, Kyoto University

The glow in the liver of this transgenic medaka fish could mean bad news =
for
the environment. In research <http://dx.doi.org/10.1021/es0486465> =
posted to
ES&T Research ASAP website this week, Japanese researchers describe how =
they
genetically engineered medaka to create the first transgenic fish =
capable of
serving as a live environmental monitor. They chose the medaka because =
it is
a small fish able to survive in fresh- and seawater over a broad =
temperature
range-handy traits for environmental monitoring. To create medaka that =
can
reveal the presence of environmental estrogens, the scientists inserted =
a
gene for green fluorescent protein (GFP) into the fish's genome so that =
the
light-emitting protein is produced in tandem with an estrogen-responsive
liver glycoprotein. Because the hatchlings seen above lack abdominal
pigmentation for 14 days, any liver fluorescence can be directly =
observed for
up to 2 weeks. The authors envision using the transgenic medaka =
hatchlings
for real-time monitoring of sewage effluents or surface waters. =
Insertion of
the GFP system into another medaka strain that remains pigment-free =
through
adulthood would allow for longer-term surveillance, suggests Masato =
Kinoshita
of Kyoto University, the corresponding author. -BARBARA BOOTH

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Laurie J. Tenace

Environmental Specialist

Florida Department of Environmental Protection

2600 Blair Stone Road, MS 4555

Tallahassee, Florida 32399-2400

PH: (850) 245-8759

FAX: (850) 245-8811

Laurie.Tenace@dep.state.fl.us

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view our mercury web pages at:=20

http://www.dep.state.fl.us/waste/categories/mercury/default.htm

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<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt'><a
href=3D"http://pubs.acs.org/subscribe/journals/esthag-w/2005/mar/tech/bb_=
estrogens.html">http://pubs.acs.org/subscribe/journals/esthag-w/2005/mar/=
tech/bb_estrogens.html</a></span></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt'>&nbsp;</span></font></p>

<p class=3DMsoNormal><font size=3D3 face=3D"Times New Roman"><span =
style=3D'font-size:
12.0pt;font-family:"Times New Roman"'>Technology News =
&#8211;</span></font></p>

<p class=3DMsoNormal><font size=3D3 face=3D"Times New Roman"><span =
style=3D'font-size:
12.0pt;font-family:"Times New Roman"'>March 9, 2005</span></font></p>

<p class=3DMsoNormal><b><font size=3D6 face=3D"Times New Roman"><span
style=3D'font-size:24.0pt;font-family:"Times New =
Roman";font-weight:bold'>Estrogens
light up genetically modified fish</span></font></b></p>

<p class=3DMsoNormal><font size=3D3 face=3D"Times New Roman"><span =
style=3D'font-size:
12.0pt;font-family:"Times New Roman"'>&nbsp;</span></font></p>

<p class=3DMsoNormal><font size=3D3 face=3D"Times New Roman"><span =
style=3D'font-size:
12.0pt;font-family:"Times New Roman"'>Masato Kinoshita, Kyoto =
University</span></font></p>

The glow in the liver of this =
transgenic
medaka fish could mean bad news for the environment. In <a
href=3D"http://dx.doi.org/10.1021/es0486465" target=3Dlink1>research</a> =
posted to ES&amp;T Research ASAP website =
this week,
Japanese researchers describe how they genetically engineered medaka to =
create
the first transgenic fish capable of serving as a live environmental =
monitor.
They chose the medaka because it is a small fish able to survive in =
fresh- and
seawater over a broad temperature range&#8212;handy traits for =
environmental
monitoring. To create medaka that can reveal the presence of =
environmental
estrogens, the scientists inserted a gene for green fluorescent protein =
(GFP)
into the fish&#8217;s genome so that the light-emitting protein is =
produced in
tandem with an estrogen-responsive liver glycoprotein. Because the =
hatchlings
seen above lack abdominal pigmentation for 14 days, any liver =
fluorescence can
be directly observed for up to 2 weeks. The authors envision using the
transgenic medaka hatchlings for real-time monitoring of sewage =
effluents or
surface waters. Insertion of the GFP system into another medaka strain =
that
remains pigment-free through adulthood would allow for longer-term
surveillance, suggests Masato Kinoshita of Kyoto University, the =
corresponding
author. &#8212;BARBARA BOOTH

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt'>&nbsp;</span></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt'>&nbsp;</span></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt'>Laurie
J. Tenace</span></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt'>Environmental
Specialist</span></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt'>Florida
Department of Environmental Protection</span></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt'>2600</span></font><font
 size=3D2><span style=3D'font-size:10.0pt'> Blair Stone Road, =
MS</span></font><font
size=3D2><span style=3D'font-size:10.0pt'> 4555</span></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt'>Tallahassee</span></font><font
 size=3D2><span style=3D'font-size:10.0pt'>, Florida =
32399-2400</span></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt'>PH:
(850) 245-8759</span></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt'>FAX:
(850) 245-8811</span></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt'><a
href=3D"mailto:Laurie.Tenace@dep.state.fl.us">Laurie.Tenace@dep.state.fl.=
us</a></span></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:11.0pt'>&nbsp;</span></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt'>view
our mercury web pages at: </span></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt'><a
href=3D"http://www.dep.state.fl.us/waste/categories/mercury/default.htm">=
http://www.dep.state.fl.us/waste/categories/mercury/default.htm</a></span=
></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:11.0pt'>&nbsp;</span></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:11.0pt'>&nbsp;</span></font></p>

<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:11.0pt'>&nbsp;</span></font></p>

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