[Pharmwaste] FW: EPA CCL3 List - Hormones, EDCs, POPs and New Emerging Polutants - trends in Europe - Happy New Year!

Tenace, Laurie Laurie.Tenace at dep.state.fl.us
Mon Jan 4 11:45:14 EST 2010


This email is being forwarded from a non-member. Although it is sent from a vendor, Florida DEP does not endorse specific equipment or companies and forwarding this email does not imply any favoritism on the part of Florida DEP. 

I hope you will find the information useful.
Laurie

-----Original Message-----
From: Peter Behnisch [mailto:Peter.Behnisch at bds.nl] 
Sent: Thursday, December 31, 2009 6:39 AM
To: kemery.dale at epa.gov; jones.enesta at epa.gov; Lyle.Milby at NormanOK.gov; SusanZ at ohanet.org; Megan.Fakih at SPARROW.ORG; stevan.gressitt at maine.gov; gilliam at adeq.state.ar.us; Tenace, Laurie; jacksonj at ebmud.com; anderson.skip at epa.gov
Cc: pharmwaste at lists.dep.state.fl.us
Subject: EPA CCL3 List - Hormones, EDCs, POPs and New Emerging Polutants - trends in Europe - Happy New Year!

Dear colleagues,

Since a few weeks I read with interest the comments on your Pharmawaste List. In this week you addressed several times the issue of the EPA-CCL3 List integrating several hormone like endocrine disrupters or we put it now here in Europe under the umbrella of  New Emerging Pollutants.

As I just come back from an European expert meeting for Pan-European Monitoring Campaigns in which we discussed how to handle such new emerging pollutants and especially the endocrine disrupting chemicals (such as hormones what you call for 14 chemicals from the CCL3 List), please let me explain our next steps forward.

We can see in the EU that mostly west-European countries can afford to analyse with sophisticated chemical detectors such new emerging pollutants in the water cycle - and  the list of such chemicals to continuously monitor gets longer and longer (EDCs, PBDEs, PFOS etc).

Now, in the EU we tried to harmonise environmental testing for water, soil, biowaste and hazardous waste with the so called "Horizontal "
projects with the aim to establish similar quality control systems than our food/feed testing - but it could not be achieved with similar guidelines, because most of the new EU countries and East-EU countries can just not afford such testing and such guidelines for all these pollutants.

Now, the aim of our expert team is to organise Pan-European sampling for water and sediments to map the pollutants all over Europe. Also for the first time the EU has established a working group for Emerging Pollutants by integrating here now also Effect-based screening methods.
Such effect-based methods cover very important endpoints (male, female hormones such as the most of the 14 chemicals listed in the CCL3 for emerging pollutants e.g. estradiol, EE2, E3) and are e.g. in the food/feed testing for dioxins/dl-PCBs are strongly recommended to be used by the European Guidelines EC/69/2002, EC/70/2002, EC/1883/2006 and
EC/152/2009 or in the US by the EPA Method 4435 called CALUX.

The advantage of using such a strategy is cost- and time- saving as well as in crisis situation you have much higher testing capacities with these high-throughput systems based on human cells.

Now, we are offering the combination of the effect-based and compound-specific analysis for some of the 14 hormone-like chemicals listed in the EPA-CCL3 list:

1.)	Effect-based analysis by BioDetection Systems (Amsterdam):

1.1.	Estrogenic and estrogenic-like hormones covering CCL3 compounds
by the ISO17025 accredited ER CALUX in vitro reportergene assay (using human cells):

such as 17alpha-estradiol; equilenin; equilin; 17-beta estradiol; Estriol; Estrone; 17-alpha ethynyl estradiol; mestranol) but as well many other chemicals such as Bisphenol A, NP, OP, DDT, some PBDEs, some phthalates and other known EDCs

1.2.) Androgenic and androgenic-like hormones by the ISO17025 accredited AR CALUX in vitro reportergene assay (using human cells): 
such hormone-like compounds are missing on the CCL3 list - What about androgens or anti-androgens like several phthalates, DDE or dieldrine..?

1.3.) Progestins by the PR CALUX: 

such as Norethindrone (19-Norethisterone)from the CCL3 list

1.4) Gluccocorticoids by GR CALUX - we find high levels of these pharmaceuticals in several hospital and industrial waste water streams (see attached EST paper from van der Linden et al (2008).

BioDetection Systems offers the CALUX hormone analysis for one endpoint such as estrogenic and estrogenic-like hormones from the CCl3 list for 

a.) water : 240 Euro (345 US$/sample);
b.) water organic solvent extracts: 170 Euro (245 US$)/sample.

In case of a 2nd CALUX analysis (such as PR CALUX for Norethindrone) it would only cost additional 80 Euro (115 US$)/sample.

Therefore you are able to measure all hormone-like compounds from the
CCL3 list for just 320 Euro (460 US$)or 250 Euro (360 US$).

In case you wish to analyse the panel of CALUX hormones such as estrogenic (ER CALUX), androgenic (AR CALUX), anti-androgenic (Anti-AR CALUX), progestenic (PR CALUX), glucocorticoid (GR CALUX) and thyroid (TR CALUX)-like compounds called CALUX-5 panel the costs would be as
following:

a.) water : 560 Euro (800 US$/sample);
b.) water organic solvent extracts: 400 Euro (575 US$)/sample.


2.)	Chemical analysis (ISO 17025 accredited partner laboratory) in
Amsterdam
2.1) Estrogenic and estrogenic-like hormones from the CCL3 compounds
list such as 17alpha-estradiol; 17-beta estradiol; Estriol; Estrone;
17-alpha ethynyl estradiol; as well as Bisphenol A: 350 Euro (500 US$)

2.2) Endocrine disrupting chemicals such as NP, OP,
di-n-octyl-phthalate, di-(2-ethylhexyl)phthalate, Tri-chlor-benzenes:
170 Euro (245 US$)

2.3) PBDEs 28 to 154: 260 Euro (375 US$. In case of further interest
please be so kind to ask for more congener specific details.

2.4) 10 Phthalates: 170 Euro (245 US$. In case of further interest
please be so kind to ask for more congener specific details.

We also would be very pleased if more colleagues and expert teams
promote the combined analysis of effect- and congener-specific analysis
by using the bridge of the EEF/EEQ model.
Only a few papers are published so far, just as Murk et al (2002),
Behnisch et al (1998) or Nakata et al (2004).

We would be very pleased to learn more about your interest and
experiences with such effect-based analysis combined with chemical
analysis.

We are looking forward to your response.

Wish you a Happy New Year!

Best wishes,

Dr. Peter A. Behnisch
BioDetection Systems
Science Park Amsterdam
Netherlands
Skype: Behnisch.bds




-----Oorspronkelijk bericht-----
Van: pharmwaste-bounces at lists.dep.state.fl.us
[mailto:pharmwaste-bounces at lists.dep.state.fl.us] Namens Lyle Milby
Verzonden: dinsdag 29 december 2009 18:29
Aan: Susan Zabo; pharmwaste at lists.dep.state.fl.us
Onderwerp: RE: [Pharmwaste] Pharmaceutical List

I did a search on the EPA's web site
http://www.epa.gov/ogwdw000/ccl/ccl3.html
for "pharmaceuticals" and came up with 14 (I don't know why the
difference) substances that were listed as either used in
pharmaceuticals or in their production:

Substance Name	CASRN		Use

17alpha-estradiol	57-91-0	It is an estrogenic hormone and is used
in pharmaceuticals.

Benzyl chloride	100-44-7	It is used in the production of other
substances, such as plastics, dyes, lubricants, gasoline and
pharmaceuticals.

equilenin	517-09-9	It is an estrogenic hormone and is used
in pharmaceuticals.

equilin	474-86-2	It is an estrogenic hormone and is used in
pharmaceuticals.

Erythromycin	114-07-8	It is used in pharmaceutical
formulations as an antibiotic.

Estradiol (17-beta estradiol)	50-28-2	It is an estrogenic hormone and
is used in pharmaceuticals.

estriol	50-27-1	It is an estrogenic hormone and is used in veterinary
pharmaceuticals.

estrone	53-16-7	It is an estrogenic hormone and is used in veterinary
and human pharmaceuticals.

Ethinyl Estradiol (17-alpha ethynyl estradiol)	57-63-6	It is an
estrogenic hormone and is used in veterinary and human pharmaceuticals

Mestranol 	72-33-3	It is an estrogenic hormone and is used in
veterinary and human pharmaceuticals.

Nitroglycerin	55-63-0	It is used in pharmaceuticals, in the production
of explosives, and in rocket propellants.

Norethindrone (19-Norethisterone)	68-22-4	It is a progresteronic
hormone used in pharmaceuticals.

o-Toluidine	95-53-4	It is used in the production of other
substances, such as dyes, rubber, pharmaceuticals and pesticides.

Quinoline	91-22-5	It is used in the production of other
substances, and as a pharmaceutical (anti-malarial) and as a flavoring
agent.

Lyle Milby
City of Norman
Environmental Services
P.O. Box 370
Norman, OK  73070
(405) 292-9731
lyle.milby at normanok.gov


-----Original Message-----
From: pharmwaste-bounces at lists.dep.state.fl.us
[mailto:pharmwaste-bounces at lists.dep.state.fl.us] On Behalf Of Susan
Zabo
Sent: Tuesday, December 29, 2009 10:01 AM
To: pharmwaste at lists.dep.state.fl.us
Subject: [Pharmwaste] Pharmaceutical List

Does anyone have the list of the 13 pharmaceuticals that the U.S.
Environmental Protection Agency put on what it calls the Contaminant
Candidate List for the first time this year. Most are hormones, but the
list also includes the antibiotic erythromycin.
thanks, susan

-------------- next part --------------
A non-text attachment was scrubbed...
Name: Murk et al (2002).pdf
Type: application/octet-stream
Size: 127079 bytes
Desc: Murk et al (2002).pdf
Url : http://lists.dep.state.fl.us/pipermail/pharmwaste/attachments/20100104/747e1a46/Murketal2002-0001.obj
-------------- next part --------------
A non-text attachment was scrubbed...
Name: Van der Linden et al (2008).pdf
Type: application/octet-stream
Size: 370843 bytes
Desc: Van der Linden et al (2008).pdf
Url : http://lists.dep.state.fl.us/pipermail/pharmwaste/attachments/20100104/747e1a46/VanderLindenetal2008-0001.obj


More information about the Pharmwaste mailing list